Recombinant p53 anticorps

N° du produit ABIN7477547

L’anticorps Lapin Monoclonal anti-p53 a été validé pour IHC. Il convient pour détecter p53 dans des échantillons de Humain.

Aperçu rapide pour Recombinant p53 anticorps (ABIN7477547)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-053R

Détail du produit anti-p53 anticorps

Immunogène: Recombinant human full-length TP53 protein

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Tonsil: More than 20 % of germinal centre B-cells must show a weak to moderate nuclear staining.

Negative Control: Colon: Luminal epithelial cells must remain p53 negative.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet: Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53,p53 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg