TP53BP1 anticorps (AA 1155-1945)

N° du produit ABIN7599769

Cet anticorps anti-TP53BP1 est un anticorps Lapin Polyclonal détectant TP53BP1 dans WB, ELISA, IF, ICC et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour TP53BP1 anticorps (AA 1155-1945) (ABIN7599769)

Antigène: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TP53BP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-TP53BP1 anticorps

Épitope: AA 1155-1945

Fonction: Anti-TP53BP1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-TP53BP1 Antibody Picoband® (ABIN7599769). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human TP53BP1 recombinant protein (Position: Q1155-E1945).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Iwabuchi, K., Bartel, P. L., Li, B., Marraccino, R., Fields, S. Two cellular proteins that bind to wild-type but not mutant p53. Proc. Nat. Acad. Sci. 91: 6098-6102, 1994. 2. Iwabuchi, K., Li, B., Massa, H. F., Trask, B. J., Date, T., Fields, S. Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2. J. Biol. Chem. 273: 26061-26068, 1998 3. Wang, B., Matsuoka, S., Carpenter, P. B., Elledge, S. J. 53BP1, a mediator of the DNA damage checkpoint. Science 298: 1435-1438, 2002.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur TP53BP1

Antigène: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Autre désignation: TP53BP1

Sujet:

Synonyms: Heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2/B1, HNRNPA2B1, HNRPA2B1

Tissue Specificity: Present in kidney, lung, testis and placenta, very low levels in liver.

Background: TP53BP1(Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al.(1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al.(1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al.(1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21(CDKN1A). Wang et al.(2002) used small interfering RNA ed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

Poids moléculaire: 300-350 kDa

ID gène: 7158

UniProt: Q12888

Pathways: Réparation de l'ADN