p53 anticorps (N235K, N239Y)

N° du produit ABIN729763

Cet anticorps Lapin Polyclonal détecte spécifiquement p53 dans ELISA, FACS, IHC (p), IF (p), IF (cc) et IHC (fro). Il présente une réactivité envers Humain et Souris et a été mentionné dans 3+ publications.

Aperçu rapide pour p53 anticorps (N235K, N239Y) (ABIN729763)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-p53 anticorps

Épitope: N235K, N239Y

Réactivité croisée: Humain, Souris

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Mtp53 around N235K N239Y

Isotype: IgG

Information d'application

Indications d'application: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-p53 (ABIN729763)

Huang, Dong, Li, Zhang, Shan, Teng: "Differential expression patterns and clinical significance of estrogen receptor-? and ? in papillary thyroid carcinoma." dans: BMC cancer, Vol. 14, pp. 383, (2014) (PubMed).

Zhao, Yao, Li, An, Chen, Sun, Kang, Wang, Hu: "Pokemon enhances proliferation, cell cycle progression and anti-apoptosis activity of colorectal cancer independently of p14(ARF)-MDM2-p53 pathway." dans: Medical oncology (Northwood, London, England), Vol. 31, Issue 12, pp. 288, (2014) (PubMed).

Zhang, Kuai, Ji, Li, Shi: "Over-Expression of Small Ubiquitin-Related Modifier-1 and Sumoylated p53 in Colon Cancer." dans: Cell biochemistry and biophysics, (2013) (PubMed).

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Sujet:

Synonyms: P53, BCC7, LFS1, TRP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53

Background: Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis, the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

ID gène: 7157

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg