Recombinant HSP27 anticorps

N° du produit ABIN7654814

L’anticorps anti-HSP27 Monoclonal Souris est utilisé pour la détection de HSP27 dans des échantillons de Humain, Souris, Rat, Singe, Mouton, Poulet et Chimpanzé.

Aperçu rapide pour Recombinant HSP27 anticorps (ABIN7654814)

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat, Singe, Mouton, Poulet, Chimpanzé

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Veuillez nous consulter SVP

Clone: RHSPB1-774

Détail du produit anti-HSP27 anticorps

Fonction: HSP27 (Heat Shock Protein 27) (rHSPB1/774)

Attributs du produit: MAb rHSPB1/774 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumorsPrimary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human HSPB1 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Commentaires:

Positive Control: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSP27

Sujet:

Synonyms: Heat shock 27 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25 kDa protein 1, Heat shock 28 kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Gene Symbol: HSPB1

Poids moléculaire: 27 kDa

ID gène: 3315

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling