Insulin anticorps

N° du produit ABIN7865657

Cet anticorps Souris Monoclonal détecte spécifiquement Insulin dans IHC, ELISA, IF, ICC et FACS. Il présente une réactivité avec des échantillons de Humain, Souris, Porc, Boeuf (Vache) et Rat.

Aperçu rapide pour Insulin anticorps (ABIN7865657)

Antigène: Insulin (INS)

Reactivité: Humain, Souris, Porc, Boeuf (Vache), Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Insulin est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 2D11-H5

Détail du produit anti-Insulin anticorps

Fonction: anti-Insulin mouse monoclonal, 2D11-H5, purified

Purification: affinity chromatography

Immunogène: purified pig insulin, conjugated to KLH

Isotype: IgG1 kappa

Information d'application

Indications d'application:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur Insulin

Antigène: Insulin (INS)

Autre désignation: Insulin

Sujet: Insulin is a protein consisting of an α-chain of 21 amino acids and a β-chain of 30 amino acids and produced in the β-cells of the pancreas. 2D11-H5 is a specific insulin antibody as tested by ELISA and on human pancreatic tissue. Positive Control: MIA, PaCa-2 cells, pancreas or insulinoma.

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation