MDM2 anticorps (pSer166)

N° du produit ABIN743753

L’anticorps Lapin Polyclonal anti-MDM2 a été validé pour WB, ELISA, IHC (p), ICC, IF (p), IF (cc), FACS et IHC (fro). Il convient pour détecter MDM2 dans des échantillons de Humain.

Aperçu rapide pour MDM2 anticorps (pSer166) (ABIN743753)

Antigène: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MDM2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-MDM2 anticorps

Épitope: pSer166

Réactivité croisée: Humain

Homologie: Mouse,Rat,Horse,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human MDM2 around the phosphorylation site of Ser166

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur MDM2

Antigène: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Autre désignation: MDM2

Sujet:

Synonyms: MDM2Ser166 , MDM2 Ser166, Double minute 2 protein, Hdm 2, HDM2, MDM 2, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein mouse binding protein 104 kDa, MDM2BP, Mouse Double Minute 2, MTBP, Murine Double Minute Chromosome 2, Oncoprotein Mdm2, p53 Binding Protein Mdm2, Ubiquitin protein ligase E3 Mdm2.

Background: Inhibits TP53/p53- and TP73/p73-mediated cell cyclearrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein(RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with TP53/p53 and WWOX. Interacts with CDKN2AIP, MTBP, TRBG1 and USP7. Isoform Mdm2-F does not interact with TP53/p53. Interacts with PYHIN1. Interacts with, and ubiquitinates HIV-1 Tat. Belongs to the MDM2/MDM4 family.

ID gène: 4193

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway