Albumin anticorps (FITC)

N° du produit ABIN784422

Détail du produit anti-Albumin anticorps (FITC)

Antigène: Albumin (ALB)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Albumin est conjugé à/à la FITC

Application: Immunofluorescence (IF), Immunodiffusion (ID), Radioimmunoassay (RIA)

Specificité: The defined antibody specificity is directed to Albumin as tested against Mouse sera. In Immunoelectrophoresis and double radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against Mouse serum and the purified Albumin. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in Double Radial Immunodiffusion with the following results: Bovine (-), Chicken (-), Canine (-), Goat (-), Guinea Pig (++), Hamster (+), Horse (+), Human (+), Monkey (+), Rat (++), Sheep (-), Swine (+).

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Attributs du produit: Absorption / Emission: 492 nm / 515 nm
Molar Ratio: Fluorescein/IgG ~1.7

Purification: Hyperimmune with strong precipitating activity are selected for fractionation by Salt Precipitation and the purification of the IgG fraction by DEAE Chromatography

Immunogène: Albumin is isolated from Mouse serum by Sequential Precipitation and purified by Ion Exchange Chromatography and Affinity Chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore by adding 1 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Détails sur Albumin

Antigène: Albumin (ALB)

Autre désignation: Albumin (ALB Produits)

Synonymes: anticorps PRO0883, anticorps PRO0903, anticorps PRO1341, anticorps ALB, anticorps CSA, anticorps Alb-1, anticorps Alb1, anticorps Albza, anticorps LOC100136344, anticorps alb-a, anticorps alb-b, anticorps albumin, anticorps serum albumin 1, anticorps albumin S homeolog, anticorps ALB, anticorps Alb, anticorps LOC100136344, anticorps alb.S

Sujet: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

ID gène: 11657

NCBI Accession: NP_033784

Pathways: Lipid Metabolism