F4/80 anticorps (FITC)

N° du produit ABIN784615

Détail du produit anti-F4/80 anticorps (FITC)

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivité: Souris, Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp F4/80 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Specificité: This antibody recognizes the F4/80 antigen on major subpopulations of resident tissue macrophages (Schaller et al. 2002). Antigen Distribution Isolated Cells: The antigen is expressed in vitro on over 80 % of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes. Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin) except lung. It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas, the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.Isolated Cells:Tissue Sections:

Réactivité croisée (Details): Species reactivity (tested):Mouse (major subpopulation of resident tissue macrophages), Human (heart macrophages)

Purification: Affinity chromatography

Immunogène: Cultured macrophages

Clone: BM8

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide, 10 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur F4/80

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Autre désignation: Macrophage F4/80 Antigen (EMR1 Produits)

Synonymes: anticorps EMR1, anticorps TM7LN3, anticorps DD7A5-7, anticorps EGF-TM7, anticorps F4/80, anticorps Gpf480, anticorps Ly71, anticorps adhesion G protein-coupled receptor E1, anticorps ADGRE1, anticorps LOC100424335, anticorps Adgre1

Sujet: The antigen expression increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to β-cell destruction and diabetes in a mouse diabetes model.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

ID gène: 13733

NCBI Accession: NP_034260

UniProt: Q61549