SLC12A1 anticorps (AA 33-55)

N° du produit ABIN863200

Détail du produit anti-SLC12A1 anticorps

Antigène: SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))

Épitope: AA 33-55

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC12A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificité: Detects ~160 kDa.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein A Purified

Immunogène: Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN863200 was sufficient for detection of NKCC2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-SLC12A1 (ABIN863200)

Hyndman, Mironova, Giani, Dugas, Collins, McDonough, Stockand, Pollock: "Collecting Duct Nitric Oxide Synthase 1ß Activation Maintains Sodium Homeostasis During High Sodium Intake Through Suppression of Aldosterone and Renal Angiotensin II Pathways." dans: Journal of the American Heart Association, Vol. 6, Issue 10, (2018) (PubMed).

Limmer, Schinner, Castrop, Vitzthum, Hofmann, Schlossmann: "Regulation of the Na(+) -K(+) -2Cl(-) cotransporter by cGMP/cGMP-dependent protein kinase I after furosemide administration." dans: The FEBS journal, (2015) (PubMed).

Zhang, Sun, Ding, Huang, Zhang, Jia: "Inhibition of Mitochondrial Complex-1 Prevents the Downregulation of NKCC2 and ENaCα in Obstructive Kidney Disease." dans: Scientific reports, Vol. 5, pp. 12480, (2015) (PubMed).

Davies, Fraser, Galic, Choy, Katerelos, Gleich, Kemp, Mount, Power: "Novel mechanisms of Na+ retention in obesity: phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK." dans: American journal of physiology. Renal physiology, Vol. 307, Issue 1, pp. F96-F106, (2014) (PubMed).

Détails sur SLC12A1

Antigène: SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))

Autre désignation: NKCC2 (SLC12A1 Produits)

Synonymes: anticorps slc12a1, anticorps SLC12A1, anticorps DKFZp469A2020, anticorps BSC1, anticorps NKCC2, anticorps Nkcc2, anticorps AI788571, anticorps D630042G03Rik, anticorps mBSC1, anticorps urehr3, anticorps si:ch211-220f12.1, anticorps solute carrier family 12 member 1, anticorps solute carrier family 12, member 1, anticorps si:ch211-220f12.1, anticorps SLC12A1, anticorps Slc12a1

Sujet: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).

ID gène: 25065

NCBI Accession: NP_001257546

UniProt: Q5PQV1