LSD1 anticorps (AA 101-200) (Alexa Fluor 647)

N° du produit ABIN903562

Détail du produit anti-LSD1 anticorps (Alexa Fluor 647)

Antigène: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Épitope: AA 101-200

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LSD1 est conjugé à/à la Alexa Fluor 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Réactivité croisée: Humain, Souris, Rat

Homologie: Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human KDM1A

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur LSD1

Antigène: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Autre désignation: KDM1A (KDM1A Produits)

Synonymes: anticorps AOF2, anticorps BHC110, anticorps KDM1, anticorps LSD1, anticorps 1810043O07Rik, anticorps AA408884, anticorps Aof2, anticorps D4Ertd478e, anticorps Kdm1, anticorps Lsd1, anticorps mKIAA0601, anticorps DKFZp459N2450, anticorps RGD1562975, anticorps aof2, anticorps kdm1, anticorps wu:fb82e06, anticorps wu:fc51a02, anticorps wu:fj36a05, anticorps wu:fj66b06, anticorps KDM1A, anticorps lsd1, anticorps xLSD1, anticorps AtLSD1, anticorps F9F13.30, anticorps F9F13_30, anticorps LESION SIMULATING DISEASE, anticorps LESION SIMULATING DISEASE 1, anticorps lysine demethylase 1A, anticorps lysine (K)-specific demethylase 1A, anticorps lysine (K)-specific demethylase 1a, anticorps LSD1 zinc finger family protein, anticorps KDM1A, anticorps Kdm1a, anticorps kdm1a, anticorps LSD1

Sujet:

Synonyms: AOF2, KDM1, LSD1, BHC110, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, KDM1A, KIAA0601

Background: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.

ID gène: 23028

UniProt: O60341

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, L'effet Warburg