PAG1 anticorps (C-Term)

N° du produit ABIN94270

Cet anticorps Souris Monoclonal détecte spécifiquement PAG1 dans WB, IP et IHC (p). Il présente une réactivité envers Humain, Souris, Rat et Boeuf (Vache) et a été mentionné dans 2+ publications.

Aperçu rapide pour PAG1 anticorps (C-Term) (ABIN94270)

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PAG1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PAG-C1

Détail du produit anti-PAG1 anticorps

Épitope: C-Term

Fonction: Anti-PAG1 Purified

Specificité: The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Réactivité croisée (Details): Mouse, Human, Rat, Bovine, Other not tested

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1.
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-PAG1 (ABIN94270)

Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." dans: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, (2003) (PubMed).

Vang, Abrahamsen, Myklebust, Horejsí, Taskén: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." dans: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, (2003) (PubMed).

Détails sur PAG1

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Autre désignation: PAG1

Sujet: Phosphoprotein membrane anchor with glycosphingoli,PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.,CBP, PAG

ID gène: 94212

UniProt: Q3U1F9

Pathways: Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events