PAG1 anticorps (AA 97-432)

N° du produit ABIN94272

Cet anticorps anti-PAG1 est un anticorps Souris Monoclonal détectant PAG1 dans WB, IHC (p) et ICFC. Adapté pour Humain. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour PAG1 anticorps (AA 97-432) (ABIN94272)

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PAG1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Intracellular Flow Cytometry (ICFC)

Clone: MEM-255

Détail du produit anti-PAG1 anticorps

Épitope: AA 97-432

Fonction: Anti-Hu PAG1 Purified

Specificité: The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).

Aucune reactivité croisée: Boeuf (Vache), Souris, Rat

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 2 μg/mL. Intracellular staining.
Immunohistochemistry (paraffin sections): Positive tissue: tonsil, spleen.
Western blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which, however, migrates on SDS-PAGE gels anomalously as an 80 kDa molecule.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-PAG1 (ABIN94272)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." dans: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Yerly, Ding, Tauzin, van Echten-Deckert, Borisch, Hoessli: "The sphingolipid-rich rafts of ALK+ lymphomas downregulate the Lyn-Cbp/PAG signalosome." dans: European journal of haematology, (2010) (PubMed).

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." dans: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).

Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." dans: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, (2003) (PubMed).

Brdicková, Brdicka, Andera, Spicka, Angelisová, Milgram, Horejsí: "Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton." dans: FEBS letters, Vol. 507, Issue 2, pp. 133-6, (2001) (PubMed).

Détails sur PAG1

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Autre désignation: PAG1

Sujet: Phosphoprotein membrane anchor with glycosphingoli,PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.,CBP, PAG

ID gène: 55824

UniProt: Q9NWQ8

Pathways: Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events