LCK anticorps (AA 22-36)

N° du produit ABIN94422

Cet anticorps anti-LCK est un anticorps Souris Monoclonal détectant LCK dans WB, IP, ICC et ICFC. Adapté pour Humain. Ce Primary Antibody a été cité dans 7+ publications.

Aperçu rapide pour LCK anticorps (AA 22-36) (ABIN94422)

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LCK est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Clone: LCK-01

Détail du produit anti-LCK anticorps

Épitope: AA 22-36

Fonction: Anti-LCK Purified

Specificité: The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase (intracellular antigen).

Aucune reactivité croisée: Souris

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Peptide corresponding to amino acids 22-36 in the sequence of human Lck.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-LCK (ABIN94422)

Stepanek, Kalina, Draber, Skopcova, Svojgr, Angelisova, Horejsi, Weiss, Brdicka: "Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148." dans: The Journal of biological chemistry, Vol. 286, Issue 25, pp. 22101-12, (2011) (PubMed).

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." dans: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Ota: "The effects of membrane compartmentalization of Csk on TCR signaling." dans: Biochimica et biophysica acta, (2010) (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." dans: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Romagnoli, Strahan, Pelosi, Cantagrel, van Meerwijk: "A potential role for protein tyrosine kinase p56(lck) in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness." dans: International immunology, Vol. 13, Issue 3, pp. 305-12, (2001) (PubMed).

Cebecauer, Cerný, Horejsí: "Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells." dans: Biochemical and biophysical research communications, Vol. 243, Issue 3, pp. 706-10, (1998) (PubMed).

Détails sur LCK

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Autre désignation: LCK

Sujet: LCK proto-oncogene, Src family tyrosine kinase,Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.,p56Lck

ID gène: 3932

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling