ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Specificité
Human ALB / Albumin. The defined antibody specificity is directed to albumin as tested against human sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against human serum and the purified albumin. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested in detail.
ALB
Reactivité: Macaque rhésus
WB, IHC, ICC, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Approved: ELISA, IHC, WB
Usage: The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e. g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays are between 1:1000 and 1:10000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Sterile distilled water 1 ml
Concentration
Lot specific
Buffer
Lyophilized from PBS, pH 7.2
Conseil sur la manipulation
Avoid repeat freeze-thaw cycles.
Stock
4 °C,-20 °C
Stockage commentaire
Long term: -20°C Short term: +4°C. Avoid repeat freeze-thaw cycles.