TRAF6 anticorps (C-Term)

N° du produit ABIN962140

Cet anticorps Lapin Polyclonal détecte spécifiquement TRAF6 dans WB, IHC, IP et IHC (p). Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour TRAF6 anticorps (C-Term) (ABIN962140)

Antigène: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRAF6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TRAF6 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Recognizes human TRAF6 proteins. On Western blots, it identifies a band at ~55kD and a band at ~60kD. The two bands may represent the two reported forms of this protein (522 and 530 amino acids).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide derived from the C-terminal region of the human and mouse TRAF6 (TNF receptor-associated factor 6) protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (1:200 - 1:400), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Immunoprecipitation: 7 μg/reaction. Positive control: Human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates. Please note that cross-reactivity of this antibody with mouse has been confirmed only in WB. The antibody has not been evaluated for use in IHC with mouse samples at this time and suitability cannot be guaranteed.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur TRAF6

Antigène: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Autre désignation: TRAF6

Sujet: Name/Gene ID: TRAF6

Synonyms: TRAF6, RING finger protein 85, MGC:3310, RNF85

ID gène: 7189

UniProt: Q9Y4K3

Pathways: Signalisation NF-kappaB, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway