WNT1 anticorps (Internal Region)

N° du produit ABIN964685

Détail du produit anti-WNT1 anticorps

Antigène: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Épitope: Internal Region

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp WNT1 est non-conjugé

Application: Western Blotting (WB)

Specificité: This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with human and mouse Wnt1 protein.  A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence.  Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology.  Cross-reactivity with Wnt1 from other sources has not been determined.

Attributs du produit: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Purification: purified

Stérilité: Sterile filtered

Immunogène: Synthetic peptide corresponding to an internal region of human Wnt1 protein
Immunogen Type: Peptide

Clone: 13F9

Isotype: IgG1 kappa

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 41 kDa in size corresponding to Wnt1 by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: WNT1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Date de péremption: 3 months

Détails sur WNT1

Antigène: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Autre désignation: Wnt1 (WNT1 Produits)

Synonymes: anticorps Xint-1, anticorps Xwnt1, anticorps int-1, anticorps int1, anticorps wnt-1, anticorps wnt1-a, anticorps Int-1, anticorps Wnt-1, anticorps sw, anticorps swaying, anticorps BMND16, anticorps INT1, anticorps OI15, anticorps Int1, anticorps WNT-1, anticorps sb:eu647, anticorps zgc:194464, anticorps zgc:194478, anticorps WNT1, anticorps Wnt family member 1, anticorps Wnt family member 1 L homeolog, anticorps wingless-type MMTV integration site family, member 1, anticorps WNT1, anticorps wnt1.L, anticorps Wnt1, anticorps wnt1

Sujet: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.
Synonyms: INT1, Proto-oncogene protein Wnt-1

ID gène: 7471, 139743

UniProt: P04628

Pathways: Signalisation WNT, Dopaminergic Neurogenesis