SMAD3 anticorps (Internal Region, pThr179)

N° du produit ABIN964922

Détail du produit anti-SMAD3 anticorps

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Épitope: Internal Region, pThr179

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein.   Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot.  Expect reactivity against phosphorylated Smad2.  Reactivity against other phosphorylated Smad family members is not known.  A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen.  Reactivity against homologues from other sources is not known.

Attributs du produit: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.

Purification: affinity purified

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. 

Commentaires:

Gene Name: SMAD3

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.97 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Date de péremption: 3 months

Références pour anticorps anti-SMAD3 (ABIN964922)

Feng, Cook, Tsai, Zhou, LaFlamme, Evans, Chen: "Discovery of a Small-Molecule BMP Sensitizer for Human Embryonic Stem Cell Differentiation." dans: Cell reports, Vol. 15, Issue 9, pp. 2063-75, (2017) (PubMed).

Pelish, Liau, Nitulescu, Tangpeerachaikul, Poss, Da Silva, Caruso, Arefolov, Fadeyi, Christie, Du, Banka, Schneider, Jestel, Zou, Si, Ebmeier, Bronson, Krivtsov, Myers, Kohl, Kung, Armstrong, Lemieux et al.: "Mediator kinase inhibition further activates super-enhancer-associated genes in AML. ..." dans: Nature, Vol. 526, Issue 7572, pp. 273-6, (2015) (PubMed).

Herhaus, Al-Salihi, Macartney, Weidlich, Sapkota: "OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3." dans: Nature communications, Vol. 4, pp. 2519, (2014) (PubMed).

Détails sur SMAD3

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Autre désignation: SMAD3 (SMAD3 Produits)

Synonymes: anticorps HSPC193, anticorps HsT17436, anticorps JV15-2, anticorps LDS1C, anticorps LDS3, anticorps MADH3, anticorps madh3, anticorps madh3a, anticorps smad3, anticorps wu:fa99e03, anticorps XSmad3, anticorps XenMLP, anticorps Xmad3, anticorps madh3-A, anticorps Madh3, anticorps AU022421, anticorps Smad 3, anticorps mad3, anticorps madh3b, anticorps zgc:92234, anticorps SMAD family member 3, anticorps SMAD family member 3a, anticorps SMAD family member 3 L homeolog, anticorps SMAD family member 3b, anticorps SMAD3, anticorps smad3a, anticorps smad3.L, anticorps Smad3, anticorps smad3b

Sujet: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.   These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway.  Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody

ID gène: 4088

NCBI Accession: NP_005893

UniProt: P84022

Pathways: Cycle Cellulaire, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy