CHEK1 anticorps (C-Term)

N° du produit ABIN965880

Cet anticorps anti-CHEK1 est un anticorps Lapin Polyclonal détectant CHEK1 dans IHC. Adapté pour Humain.

Aperçu rapide pour CHEK1 anticorps (C-Term) (ABIN965880)

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK1 est non-conjugé

Application: Immunohistochemistry (IHC)

Détail du produit anti-CHEK1 anticorps

Épitope: C-Term

Purification: Purified by antigen-specific affinity chromatography.

Immunogène: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human CHEK1(Serine/threonine-protein kinase Chk1)

Information d'application

Indications d'application: ELISA, Western blotting: 1µg/ml for 2hrs.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: This antibody is stored in PBS, 50% glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur CHEK1

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: CHEK1

Sujet: CHEK1(Serine/threonine-protein kinase Chk1) involved in cell cycle arrest when dna damage has occurred or when unligated dna is present. CHEK1 binds to and phosphorylates CDC25A, CDC25B and CDC25C. The phosphorylation of CDC25C creates a binding site for 14-3-3 protein which inhibits CDC25C. This prevents activation of the CDC2-Cyclin B complex and prevents mitotic entry. The CHEK1 phosphorylates CDC25C on serine216. Chk1 plays a primary role in the protection of cells from death induced by replication fork stress, whereas p21 mediates through its role in regulating entry into S phase. Chk1 deficiency resulted in a premature onset of mitosis because of abnormal activation of cyclin B-Cdc2 and led to the activation of caspases 3 and 9 triggered by cytoplasmic release of cytochrome c. It belongs to the Ser/Thr family of protein kinases and NIM1 subfamily.

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN