XRCC6 anticorps (AA 506-541)

N° du produit ABIN966469

Détail du produit anti-XRCC6 anticorps

Antigène: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Épitope: AA 506-541

Reactivité: Humain, Souris, Singe, Xenopus laevis, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp XRCC6 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purification: Concentrated. Purified from the ascites fluid by Protein A chromatography

Immunogène: Human placental extract designated as PSE1-PL

Clone: N3H10

Isotype: IgG2b

Information d'application

Indications d'application: Immunofluorescence
Immunoprecipitation (Native and denatured)(Use Protein A) (Ab 2µg/mg protein lysate)
Western blotting: (0.25-0.5 µg/ml for 2hrs at RT)
Immunohistology (Formalinparaffin)(Ab 1-2g/ml for 30 minute at RT)
Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH 6.0.

Commentaires:

Cellular Localization: Nuclear.
Recommended Positive Control: BT474, HEP-G-2 or HeLa Cells, Tonsil

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL.

Buffer: 10mM PBS, pH 7.4, with 0.2% BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-XRCC6 (ABIN966469)

Wang, Chou, Blankson, Satoh, Knuth, Eisenberg, Pisetsky, Reeves: "Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens." dans: Molecular biology reports, Vol. 18, Issue 1, pp. 15-28, (1993) (PubMed).

Détails sur XRCC6

Antigène: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Autre désignation: Ku (p70) (XRCC6 Produits)

Synonymes: anticorps CTC75, anticorps CTCBF, anticorps G22P1, anticorps KU70, anticorps ML8, anticorps TLAA, anticorps 70kDa, anticorps G22p1, anticorps Ku70, anticorps Kup70, anticorps X-ray repair cross complementing 6, anticorps ATP-dependent DNA helicase II, 70 kDa subunit, anticorps X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog, anticorps X-ray repair complementing defective repair in Chinese hamster cells 6, anticorps XRCC6, anticorps Bm1_41430, anticorps xrcc6.L, anticorps Xrcc6

Sujet: The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Also available without BSA and azide at 1mg/ml.

Poids moléculaire: 70kDa

ID gène: 2547

Pathways: Réparation de l'ADN