PARP1 anticorps (Asp214, cleaved)

N° du produit ABIN967365

Cet anticorps anti-PARP1 est un anticorps Souris Monoclonal détectant PARP1 dans WB, IP et ICS. Adapté pour Humain. Ce Primary Antibody a été cité dans 6+ publications.

Aperçu rapide pour PARP1 anticorps (Asp214, cleaved) (ABIN967365)

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PARP1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Intracellular Staining (ICS)

Clone: F21-852

Détail du produit anti-PARP1 anticorps

Épitope: Asp214, cleaved

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human cleaved PARP

Isotype: IgG1 kappa

Information d'application

Indications d'application: Camptothecin treated Jurkat lysate [50 µg (1 µg/µl)] is provided as a positive control (51-16606N, store lysate at -20°C). Additional Jurkat lysate is available untreated (ABIN968537) or as a set containing both untreated and campotothecin treated lysates (ABIN967299) as ready-to-use western blot controls. Additional applications which are not not routinely tested include immunoprecipitation (2 µg/300 µg of lysates) and flow cytometry. The directly conjugated formats of the clone are recommended for flow cytometry.

Commentaires:

Related Products: ABIN968537, ABIN967299, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the antibody at 4°C. Store the lysate at -20°C.

Références pour anticorps anti-PARP1 (ABIN967365)

DAmours, Desnoyers, DSilva, Poirier: "Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions." dans: The Biochemical journal, Vol. 342 ( Pt 2), Issue 5691, pp. 249-68, (1999) (PubMed).

Patel, Gores, Kaufmann: "The role of proteases during apoptosis." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 10, Issue 5, pp. 587-97, (1996) (PubMed).

Tewari, Quan, ORourke, Desnoyers, Zeng, Beidler, Poirier, Salvesen, Dixit: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." dans: Cell, Vol. 81, Issue 5, pp. 801-9, (1995) (PubMed).

Kaufmann, Desnoyers, Ottaviano, Davidson, Poirier: "Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis." dans: Cancer research, Vol. 53, Issue 17, pp. 3976-85, (1993) (PubMed).

Lamarre, Talbot, de Murcia, Laplante, Leduc, Mazen, Poirier: "Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study." dans: Biochimica et biophysica acta, Vol. 950, Issue 2, pp. 147-60, (1988) (PubMed).

Lamarre, Talbot, Leduc, Muller, Poirier: "Production and characterization of monoclonal antibodies specific for the functional domains of poly(ADP-ribose) polymerase." dans: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 64, Issue 4, pp. 368-76, (1986) (PubMed).

Détails sur PARP1

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Autre désignation: PARP

Sujet: PARP (Poly [ADP-Ribose] Polymerase) is a 113-kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA damage. Additionally, PARP is a target of the caspase protease activity associated with apoptosis. The PARP protein consists of an N-terminal DNA-binding domain (DBD) and a C-terminal catalytic domain separated by a central automodification domain. During apoptosis, Caspase-3 cleaves PARP at a recognition site (Asp Glu Val Asp Gly) in the DBD to form 24- and 89-kDa fragments. This process separates the DBD (which is mostly in the 24-kDa fragment) from the catalytic domain (in the 89-kDa fragment) of the enzyme, resulting in the loss of normal PARP function. It has been proposed that inactivation of PARP directs DNA-damaged cells to undergo apoptosis rather than necrotic degradation, and the presence of the 89-kDa PARP cleavage fraction is considered to be a marker of apoptosis.
A peptide corresponding to the N-terminus of the cleavage site (Asp 214) of human PARP was used as the immunogen. The F21-852 monoclonal antibody reacts only with the 89-kDa fragment of human PARP-1 that is downstream of the Caspase-3 cleavage site (Asp214) and contains the automodification and catalytic domains. It does not react with intact human PARP-1. Cross-reactivity with other members of the PARP superfamily is unknown. It may also recognize cleaved PARP in a number of other species due to the conserved nature of the molecule, although this has not been tested.

Pathways: Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location