MCL-1 anticorps (C-Term)

N° du produit ABIN967637

L’anticorps Souris Monoclonal anti-MCL-1 a été validé pour WB et IP. Il convient pour détecter MCL-1 dans des échantillons de Humain. Il y a 6+ publications disponibles.

Aperçu rapide pour MCL-1 anticorps (C-Term) (ABIN967637)

Antigène: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCL-1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 22-Mcl

Détail du produit anti-MCL-1 anticorps

Épitope: C-Term

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Purified recombinant human Mcl-1 lacking the C-terminal hydrophobic domain

Isotype: IgG1 kappa

Information d'application

Indications d'application: The antibody is tested by Western blot on K-562 cell lysate (ATCC CCL-243). Other applications include immunoprecipitation (1-2 µg/ml).

Commentaires:

Related Products: ABIN968586, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Références pour anticorps anti-MCL-1 (ABIN967637)

Gross, McDonnell, Korsmeyer: "BCL-2 family members and the mitochondria in apoptosis." dans: Genes & development, Vol. 13, Issue 15, pp. 1899-911, (1999) (PubMed).

Wang, Chao, Chen, Kuo, Yen, Yang-Yen: "The antiapoptotic gene mcl-1 is up-regulated by the phosphatidylinositol 3-kinase/Akt signaling pathway through a transcription factor complex containing CREB." dans: Molecular and cellular biology, Vol. 19, Issue 9, pp. 6195-206, (1999) (PubMed).

Adams, Cory: "The Bcl-2 protein family: arbiters of cell survival." dans: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1322-6, (1998) (PubMed).

Yang, Kozopas, Craig: "The intracellular distribution and pattern of expression of Mcl-1 overlap with, but are not identical to, those of Bcl-2." dans: The Journal of cell biology, Vol. 128, Issue 6, pp. 1173-84, (1995) (PubMed).

Kozopas, Yang, Buchan, Zhou, Craig: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, (1993) (PubMed).

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." dans: Cell, Vol. 74, Issue 4, pp. 609-19, (1993) (PubMed).

Détails sur MCL-1

Antigène: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Autre désignation: Mcl-1

Sujet: The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules that have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Mcl-1 is a member of the anti-apoptotic Bcl-2 group of proteins, including Bcl-x, Bcl-w, A1/Bfl-1, Boo/Diva, and Nr-13. Mcl-1 was cloned from the ML-1 human myeoblastic leukemia cell line. Mcl-1 localizes to the mitochrondria, like Bcl-2, but also associates with membranes via its C-terminal hydrophobic domain. Further studies have shown that Mcl-1 is upregulated in response to the GM-CSF and IL-3 cytokine signaling pathways and that this regulation of Mcl-1 is controlled at the level of transcription. Mcl-1 is detected at approximately 40 kDa in SDS/PAGE.

Poids moléculaire: 40 kDa

Pathways: Signalisation MAPK