AP2B1 anticorps (AA 75-245)

N° du produit ABIN967930

Détail du produit anti-AP2B1 anticorps

Antigène: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Épitope: AA 75-245

Reactivité: Humain, Souris, Rat, Chien, Poulet, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AP2B1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), BioImaging (BI)

Réactivité croisée: Souris, Rat (Rattus), Chien, Poulet, Grenouille

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Adaptin beta aa. 75-245

Clone: 74-Adaptin beta

Isotype: IgG1

Information d'application

Indications d'application: Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-AP2B1 (ABIN967930)

Naga Prasad, Laporte, Chamberlain, Caron, Barak, Rockman: "Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex." dans: The Journal of cell biology, Vol. 158, Issue 3, pp. 563-75, (2002) (PubMed).

Ros-Baro, Lopez-Iglesias, Peiro, Bellido, Palacin, Zorzano, Camps: "Lipid rafts are required for GLUT4 internalization in adipose cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 21, pp. 12050-5, (2001) (PubMed).

Laporte, Oakley, Zhang, Holt, Ferguson, Caron, Barak: "The beta2-adrenergic receptor/betaarrestin complex recruits the clathrin adaptor AP-2 during endocytosis." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3712-7, (1999) (PubMed).

Ponnambalam, Robinson, Jackson, Peiperl, Parham: "Conservation and diversity in families of coated vesicle adaptins." dans: The Journal of biological chemistry, Vol. 265, Issue 9, pp. 4814-20, (1990) (PubMed).

Détails sur AP2B1

Antigène: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Autre désignation: Adaptin beta (AP2B1 Produits)

Synonymes: anticorps 5-HT(2B), anticorps 5-HT2B, anticorps ADTB2, anticorps AP105B, anticorps AP2-BETA, anticorps CLAPB1, anticorps 1300012O03Rik, anticorps AI788979, anticorps fa16e07, anticorps wu:fa16c06, anticorps wu:fa16e07, anticorps wu:fc18c08, anticorps zgc:55659, anticorps 5-hydroxytryptamine receptor 2B, anticorps adaptor related protein complex 2 beta 1 subunit, anticorps adaptor-related protein complex 2, beta 1 subunit, anticorps adaptor related protein complex 2 beta 1 subunit L homeolog, anticorps HTR2B, anticorps AP2B1, anticorps Ap2b1, anticorps ap2b1, anticorps ap2b1.L

Sujet: Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (gamma, alpha, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain (sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. beta Adaptin subunits (beta1, beta2, beta3, beta4) lack sequence homology to adaptins alpha, gamma, delta, and epsilon, but all of these subunits share a similar domain structure. Adaptin beta1 (also known as Adaptin beta') and beta2 (also known as Adaptin beta) have 83% amino acid identity and are found in the AP1 and AP2 complexes, respectively.

Poids moléculaire: 106 kDa

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation