SLC9A1 anticorps (AA 682-801)

N° du produit ABIN968659

Détail du produit anti-SLC9A1 anticorps

Antigène: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Épitope: AA 682-801

Reactivité: Humain, Rat, Souris, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SLC9A1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Humain, Souris, Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat NHE-1 aa. 682-801

Clone: 54-NHE

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-SLC9A1 (ABIN968659)

Zhao, Oxley, Smith, Follows, Green, Alexander: "DNA damage-induced Bcl-xL deamidation is mediated by NHE-1 antiport regulated intracellular pH." dans: PLoS biology, Vol. 5, Issue 1, pp. e1, (2007) (PubMed).

Aharonovitz, Zaun, Balla, York, Orlowski, Grinstein: "Intracellular pH regulation by Na(+)/H(+) exchange requires phosphatidylinositol 4,5-bisphosphate." dans: The Journal of cell biology, Vol. 150, Issue 1, pp. 213-24, (2000) (PubMed).

Cox, Lutz, Yang, Biemesderfer, Bronson, Fu, Aronson, Noebels, Frankel: "Sodium/hydrogen exchanger gene defect in slow-wave epilepsy mutant mice." dans: Cell, Vol. 91, Issue 1, pp. 139-48, (1997) (PubMed).

Kandasamy, Yu, Harris, Boucher, Hanrahan, Orlowski: "Plasma membrane Na+/H+ exchanger isoforms (NHE-1, -2, and -3) are differentially responsive to second messenger agonists of the protein kinase A and C pathways." dans: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29209-16, (1996) (PubMed).

Orlowski, Kandasamy, Shull et al.: "Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally ..." dans: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9331-9, (1992) (PubMed).

Détails sur SLC9A1

Antigène: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Autre désignation: NHE-1 (SLC9A1 Produits)

Synonymes: anticorps AT-NHX1, anticorps ATNHX, anticorps ATNHX1, anticorps NA(+)/H(+) ANTIPORTER, anticorps Na+/H+ exchanger 1, anticorps T21B4.60, anticorps T21B4_60, anticorps AW554487, anticorps Apnh, anticorps Nhe1, anticorps swe, anticorps NHE-1, anticorps NHE1, anticorps APNH, anticorps Na+/H+ exchanger 1, anticorps solute carrier family 9 (sodium/hydrogen exchanger), member 1, anticorps solute carrier family 9 member A1, anticorps NHX1, anticorps Slc9a1, anticorps SLC9A1

Sujet: The extrusion of H+ in exchange for extracellular Na+ is important for many cellular processes, such as pH homeostasis, volume regulation, and transepithelial ion and water transport. Na+/H+ Exchangers (NHE) are integral membrane proteins that mediate electroneutral exchange of one Na+ ion for one H+ ion. Six NHE forms, NHE-1 thru -6, have been identified. NHE-1 and NHE-6 are widely expressed, while the other NHE forms have restricted expression. The common structure of all NHE forms includes 10-12 N-terminal membrane (M) spanning regions, a conserved M6 and M7 region that may participate in ion transport, and a large C-terminal cytoplasmic region that may be involved in the regulation of ion exchange activity. NHE-1, for example, contains 12 M regions plus domains for volume sensitivity, calmodulin-binding, CHP-binding, and PKC phosphorylation in the cytoplasmic region. Regulation of NHE-1 ion exchange activity may occur through phosphoinositide binding, as well as PKC- and PKA-dependent signaling pathways. Mutation of NHE-1 in mice causes neuronal death in the cerebellum and brainstem, leading to ataxia and seizures. Thus, NHE-1 is a ubiquitous NHE that is essential for normal brain function.
Although this antibody was developed against the NHE-1 antigen, investigators should note that crossreactivity to other NHE isoforms or variants may be possible.
Synonyms: Na+/H+ Exchangers

Poids moléculaire: 92 kDa

Pathways: Glycosaminoglycan Metabolic Process, Proton Transport