LCK anticorps (pTyr505)

N° du produit ABIN968844

Cet anticorps anti-LCK est un anticorps Souris Monoclonal détectant LCK dans WB et FACS. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour LCK anticorps (pTyr505) (ABIN968844)

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LCK est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 4-Lck

Détail du produit anti-LCK anticorps

Épitope: pTyr505

Réactivité croisée: Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Lck (pY505)

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN968656, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-LCK (ABIN968844)

Wang, Lemay, Tsai, Veillette: "SH2 domain-mediated interaction of inhibitory protein tyrosine kinase Csk with protein tyrosine phosphatase-HSCF." dans: Molecular and cellular biology, Vol. 21, Issue 4, pp. 1077-88, (2001) (PubMed).

Hardwick, Sefton: "The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505." dans: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25429-32, (1997) (PubMed).

Lee-Fruman, Collins, Burakoff: "Role of the Lck Src homology 2 and 3 domains in protein tyrosine phosphorylation." dans: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25003-10, (1996) (PubMed).

Détails sur LCK

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Autre désignation: Lck

Sujet: Protein tyrosine phosphorylation is an essential step in the signal transduction cascade leading to T cell antigen receptor (TCR) activation. Lck is a protein kinase and a member of the src family of cytoplasmic protein-tyrosine kinases (PTKs). Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. CD4 and CD8 bind to surface MHC class II and class I molecules, respectively. Lck is regulated by both kinases and phosphatases. Autophosphorylation at Y394 leads to conformational changes in the catalytic domain, which induces kinase activity. Repression of Lck occurs via phosphorylation at Y505, located near the carboxy-terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs. Upon phosphorylation at this site, Lck associates with the SH2 domain in the amino-terminus, thus keeping the protein biologically inactive. Lck activity and regulation is critical for activation and development of T cells.

Poids moléculaire: 56 kDa

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling