E-cadherin anticorps

N° du produit ABIN969036

Cité dans 3 publications. Cet anticorps anti-E-cadherin Monoclonal Souris (Clone 7H12) (ABIN969036) détecte spécifiquement E-cadherin dans WB, IHC, FACS et ELISA. L’anticorps est réactif avec des échantillons de Humain, Souris et Singe.

Aperçu rapide pour E-cadherin anticorps (ABIN969036)

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivité: Humain, Souris, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp E-cadherin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Clone: 7H12

Détail du produit anti-E-cadherin anticorps

Fonction: CDH1 Antibody

Purification: Ascitic fluid

Immunogène: Purified recombinant fragment of human CDH1 expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Références pour anticorps anti-E-cadherin (ABIN969036)

Schietke, Warnecke, Wacker, Schödel, Mole, Campean, Amann, Goppelt-Struebe, Behrens, Eckardt, Wiesener: "The lysyl oxidases LOX and LOXL2 are necessary and sufficient to repress E-cadherin in hypoxia: insights into cellular transformation processes mediated by HIF-1." dans: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6658-69, (2010) (PubMed).

Barrett, Lee, Lees, Prescott, Anderson, Phillips, Wesley, Parnell, Zhang, Drummond, Nimmo, Massey, Blaszczyk, Elliott, Cotterill, Dallal, Lobo, Mowat, Sanderson, Jewell, Newman, Edwards, Ahmad et al.: "Genome-wide association study of ulcerative colitis identifies three new susceptibility loci, including the HNF4A region. ..." dans: Nature genetics, Vol. 41, Issue 12, pp. 1330-4, (2009) (PubMed).

Wang, Yao, Zhang, Zhang, Zhao: "[Expression and significance of CD147 and E-cadherin in human gastric carcinoma]." dans: Zhonghua zhong liu za zhi [Chinese journal of oncology], Vol. 31, Issue 7, pp. 515-9, (2009) (PubMed).

Détails sur E-cadherin

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Autre désignation: CDH1

Sujet: E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.Tissue specificity: Non-neural epithelial tissues.

Poids moléculaire: 135 kDa

ID gène: 999

UniProt: P12830

Pathways: Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation