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|Antigène||Cyclin D3 (CCND3) Anticorps|
|Conjugué||Cet anticorp Cyclin D3 est non-conjugé Alternatives|
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
Détail du produit anti-Cyclin D3 anticorpsDétail du antigène Cyclin D3 Information d'application Stockage Images
|Attributs du produit||
Synonyms: rabbit anti-Cyclin D3 antibody, G1/S-specific cyclin-D3, Ccnd3, Cyl-3
Background: Anti-Cyclin D3 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Cyclin D3 belongs to a highly conserved cyclin family, whose members are the ultimate recipients of oncogenic signals. Cyclin D3 is a key component of the cell cycle progression machinery and induces progression through the G1 phase of the cell cycle. Cyclin D3 is expressed in nearly all proliferating cells, and shows the most broad expression pattern of all three D-type (D1-D3) cyclins. Cyclin D3 is encoded from the 6p21 chromosome region and the protein is predominantly localized in the nucleus. Once induced, cyclin D3 binds and activates its associated cyclin-dependent kinases CDK4 and CDK6. Amplification of the cyclin D3 gene and overexpression of cyclin D3 protein is seen in several human cancers. A large number of human malignancies contain lesions in pathways impacting on cyclin D3. Abnormal expression of Cyclin D3 is believed to be a driving force in several human cancers. A possible role for cyclin D3 in the malignancies of the lymphoid system is suggested by the observations that cyclin D3 gene is rearranged in several neoplastic diseases such as diffuse large B cell lymphomas or multiple myelomas. Anti-Cyclin D3 is ideal for researchers interested in Cancer Research and Immunology research.
Gene Name: Ccnd3
|Purification||Cyclin D3 affinity-purified antibody is directed against Cyclin D3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, and bovine sources based on 100% homology for the immunogen sequence. Cross reactivity with other sources has not been determined. No reactivity was observed against Cyclin D2.|
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.
Immunogen Type: Peptide
|Plasmids, Primers & others|
Détail du antigène Cyclin D3Détail du produit anti-Cyclin D3 anticorps Information d'application Stockage Images Haut de la page
|Autre désignation||Cyclin D3 (CCND3 Antibody Extrait)|
|Pathways||Cycle Cellulaire, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process|
Information d'applicationDétail du produit anti-Cyclin D3 anticorps Détail du antigène Cyclin D3 Stockage Images Haut de la page
Flow Cytometry Dilution: 4 μg/mL
Immunohistochemistry Dilution: 4 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, flow cytometry, and by immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 32.4 kDa in size corresponding to Cyclin D3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Cyclin D3.
Western Blot Dilution: 1.0 μg/mL
Immunoprecipitation Dilution: 5 μg/mL
ELISA Dilution: 1:1000 - 1:5000
|Restrictions||For Research Use only|
StockageDétail du produit anti-Cyclin D3 anticorps Détail du antigène Cyclin D3 Information d'application Images Haut de la page
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
|Agent conservateur||Sodium azide|
|Précaution d'utilisation||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Stock||4 °C,-20 °C|
|Date de péremption||12 months|