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Apelin Kit ELISA

APLN Reactivité: Humain Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum
N° du produit ABIN1568428
  • Antigène Voir toutes Apelin (APLN) Kits ELISA
    Apelin (APLN)
    Reactivité
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    15.625 pg/mL - 1000 pg/mL
    Seuil minimal de détection
    15.625 pg/mL
    Application
    ELISA
    Fonction
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of APLN in human serum, plasma and other biological fluids.
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of this index.
    Réactivité croisée (Details)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    Sensibilité
    6.13 pg/mL
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Volume d'échantillon
    50 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C
    3. Aspirate and wash 3 times
    4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    5. Aspirate and wash 5 times
    6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    7. Add 50µL Stop Solution. Read at 450 nm immediately.
    Procédure de l'essai

    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.

    Précision du teste
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Date de péremption
    12 months
  • Antigène Voir toutes Apelin (APLN) Kits ELISA
    Apelin (APLN)
    Autre désignation
    APLN (APLN Produits)
    Synonymes
    APLN Kit ELISA, Xapelin Kit ELISA, xnpep2 Kit ELISA, preproapelin Kit ELISA, 6030430G11Rik Kit ELISA, Apel Kit ELISA, APEL Kit ELISA, XNPEP2 Kit ELISA, apelin Kit ELISA, apelin S homeolog Kit ELISA, apln Kit ELISA, APLN Kit ELISA, apln.S Kit ELISA, Apln Kit ELISA
    Sujet
    Alternative name: XNPEP2, APEL, APJ endogenous ligand
    ID gène
    8862
    UniProt
    Q9ULZ1
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Feeding Behaviour
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