TNFSF13 Kit ELISA

N° du produit ABIN1672837

Détail du produit TNFSF13 Kit ELISA

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Épitope: AA 105-250

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFSF13/APRIL

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO,A105-L250

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A105-L250

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: human TNFSF13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF13 has been precoated onto 96-well plates. Standards (NSO,A105-L250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFSF13 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TNFSF13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFSF13 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 653, Standard deviation: 26.8, CV(%): 4.1
• Sample 2: n=16, Mean(pg/ml): 2945, Standard deviation: 156.1, CV(%): 5.3
• Sample 3: n=16, Mean(pg/ml): 5633, Standard deviation: 219.7, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 864, Standard deviation: 42.4, CV(%): 4.9
• Sample 2: n=24, Mean(pg/ml): 3214, Standard deviation: 202.5, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 5366, Standard deviation: 268.3, CV(%): 5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour TNFSF13 Kit ELISA (ABIN1672837)

Gözmen, Karapnar, Tüfekçi, Vergin, Yüksel, Irken, Oren: "B-cell-activating factor, a proliferation inducing ligand and co-stimulatory molecules in the pathogenesis of immune thrombocytopenia in childhood." dans: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, (2014) (PubMed).

Détail du antigène

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Autre désignation: TNFSF13/APRIL (TNFSF13 Produits)

Synonymes: APRIL Kit ELISA, CD256 Kit ELISA, TALL-2 Kit ELISA, TALL2 Kit ELISA, TRDL-1 Kit ELISA, ZTNF2 Kit ELISA, 2310026N09Rik Kit ELISA, April Kit ELISA, Tall2 Kit ELISA, Trdl1 Kit ELISA, SLAN Kit ELISA, ANP32B Kit ELISA, april Kit ELISA, tnf13 Kit ELISA, TNLG7B Kit ELISA, TNF superfamily member 13 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 13 Kit ELISA, acidic leucine-rich nuclear phosphoprotein 32 family member B Kit ELISA, TNFSF13 Kit ELISA, Tnfsf13 Kit ELISA, LOC494442 Kit ELISA, tnfsf13 Kit ELISA

Sujet:

Background: Tumor necrosis factor ligand superfamily member 13(TNFSF13) also known as a proliferation-inducing ligand(APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256(cluster of differentiation 256). The protein encoded by this gene is a member of the tumor necrosis factor ligand(TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.

Synonyms: Tumor necrosis factor (Ligand) superfamily member 13 transcript variant delta ,Tumor necrosis factor ligand superfamily member 13 ,TNFSF13 ,

Full Gene Name: tumor necrosis factor (ligand) superfamily, member 13

ID gène: 8741

UniProt: Q2QBA2

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response