TNFSF13 Kit ELISA

N° du produit ABIN6720117

Détail du produit TNFSF13 Kit ELISA

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: A proliferation-inducing ligand, APRIL, CD256, TALL-2, TALL2, TNF- and APOL-related leukocyte expressed ligand 2, TNF-related death ligand 1, TNF13_HUMAN, TNFSF13, TRDL-1, Tumor necrosis factor (ligand) superfamily member 13, Tumor necrosis factor ligand superfamily member 13, Tumor necrosis factor like protein ZTNF2, Tumor necrosis factor related death ligand 1, ZTNF2

Background: Tumor necrosis factor ligand superfamily member 13(TNFSF13) also known as a proliferation-inducing ligand(APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256(cluster of differentiation 256). The protein encoded by this gene is a member of the tumor necrosis factor ligand(TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.

Gene Name: TNFSF13

Production: Natural and recombinant human TNFSF13. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A105-L250

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TNFSF13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TNFSF13 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Autre désignation: TNFSF13 - APRIL (TNFSF13 Produits)

Synonymes: APRIL Kit ELISA, CD256 Kit ELISA, TALL-2 Kit ELISA, TALL2 Kit ELISA, TRDL-1 Kit ELISA, ZTNF2 Kit ELISA, 2310026N09Rik Kit ELISA, April Kit ELISA, Tall2 Kit ELISA, Trdl1 Kit ELISA, SLAN Kit ELISA, ANP32B Kit ELISA, april Kit ELISA, tnf13 Kit ELISA, TNLG7B Kit ELISA, TNF superfamily member 13 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 13 Kit ELISA, acidic leucine-rich nuclear phosphoprotein 32 family member B Kit ELISA, TNFSF13 Kit ELISA, Tnfsf13 Kit ELISA, LOC494442 Kit ELISA, tnfsf13 Kit ELISA

ID gène: 8741

NCBI Accession: NP_001185551

UniProt: Q2QBA2

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response