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PDGF-AB Heterodimer Kit ELISA

Reactivité: Souris Colorimetric Sandwich ELISA 31.2-2000 pg/mL
N° du produit ABIN2042633
  • Antigène
    PDGF-AB Heterodimer
    Reactivité
    • 6
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Marque
    BioAssay™
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    < 3 pg/mL
    Ingrédients
    96-well plate pre-coated with anti-PDGF-AB, Mouse
    Lyophilized recombinant PDGF-AB, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
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  • Plaque
    Pre-coated
    Protocole
    Sandwich ELISA quantitative detection kit for Mouse PDGF-AB in cell culture supernates, serum and plasma (heparin, EDTA).The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.
    Restrictions
    For Research Use only
  • Stock
    4 °C/-20 °C
  • Antigène
    PDGF-AB Heterodimer
    Autre désignation
    PDGF-AB
    Synonymes
    PDGF-A Kit ELISA, PDGF1 Kit ELISA, platelet derived growth factor subunit A Kit ELISA, PDGFA Kit ELISA
    ID gène
    18591
    UniProt
    P31240, P20033
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