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PDGF-AB Heterodimer Kit ELISA

Reactivité: Rat Colorimetric Sandwich ELISA 31.2-2000 pg/mL

N° du produit ABIN2042634

Fiche de données

Antigène

PDGF-AB Heterodimer
Reactivité
6

5

5

2

2

2

2

1

1

1

1

1
Rat

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Gamme de detection

31.2-2000 pg/mL

Seuil minimal de détection

31.2 pg/mL

Application

ELISA

Marque

BioAssay™

Réactivité croisée (Details)

There is no detectable cross-reactivity with other relevant proteins.

Sensibilité

< 3 pg/mL

Ingrédients

96-well plate pre-coated with anti-PDGF-AB, Rat
Lyophilized recombinant PDGF-AB, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

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Plaque

Pre-coated

Protocole

Sandwich ELISA quantitative detection kit for Rat PDGF-AB in cell culture supernates, serum and plasma (heparin, EDTA).The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5 The standard product used in this kit is recombinant rat PDGF-AB(A chain Ser87-Arg196, B chain Ser74-Thr182) with the molecular mass of 24.8kD.

Restrictions

For Research Use only
Stock

4 °C/-20 °C
Antigène

PDGF-AB Heterodimer

Autre désignation

PDGF-AB

Synonymes

PDGF-A Kit ELISA, PDGF1 Kit ELISA, platelet derived growth factor subunit A Kit ELISA, PDGFA Kit ELISA

ID gène

24628

UniProt

G3V882, P28576