alpha 2 Macroglobulin Kit ELISA
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- Antigène Voir toutes alpha 2 Macroglobulin (A2M) Kits ELISA
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 4-3.000 ng/mL
- Seuil minimal de détection
- 4 ng/mL
- Application
- ELISA
- Fonction
- Human Alpha-2 Macroglobulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair detects human Alpha 2-M. Other species not determined.
- Sensibilité
- 4 ng/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2,000 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes alpha 2 Macroglobulin (A2M) Kits ELISA
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
- Autre désignation
- alpha 2 M (A2M Produits)
- Synonymes
- A2MD Kit ELISA, CPAMD5 Kit ELISA, FWP007 Kit ELISA, S863-7 Kit ELISA, A2MAC1 Kit ELISA, A2m1 Kit ELISA, A2maa Kit ELISA, Mam Kit ELISA, A2mp Kit ELISA, A2M Kit ELISA, LOC733429 Kit ELISA, endod Kit ELISA, cpamd5 Kit ELISA, fwp007 Kit ELISA, s863-7 Kit ELISA, a2mb Kit ELISA, endodermin Kit ELISA, Alpha-2-M Kit ELISA, alpha-2-macroglobulin Kit ELISA, pregnancy-zone protein L homeolog Kit ELISA, alpha-2-macroglobulin S homeolog Kit ELISA, pregnancy zone protein Kit ELISA, alpha-2-macroglobulin-like Kit ELISA, A2M Kit ELISA, A2m Kit ELISA, LOC477699 Kit ELISA, pzp.L Kit ELISA, AZL_c00450 Kit ELISA, LOC100349077 Kit ELISA, a2m Kit ELISA, LOC100061656 Kit ELISA, LOC100090399 Kit ELISA, a2m.S Kit ELISA, LOC100469973 Kit ELISA, LOC100595735 Kit ELISA, PZP Kit ELISA, LOC100353095 Kit ELISA, LOC101122940 Kit ELISA, LOC101801552 Kit ELISA, LOC100911545 Kit ELISA
- Sujet
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Gene Names: A2M CPAMD5 FWP007
Protein names: Alpha-2-macroglobulin (Alpha-2-M) (C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5)
- ID gène
- 2
- UniProt
- P01023
- Pathways
- Lipid Metabolism
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