FGF23 Kit ELISA
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- Antigène Voir toutes FGF23 Kits ELISA
- FGF23 (Fibroblast Growth Factor 23 (FGF23))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.3-75 ng/mL
- Seuil minimal de détection
- 0.3 ng/mL
- Application
- ELISA
- Fonction
- Human FGF-23 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Human FGF-23
- Sensibilité
- 0.3 ng/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes FGF23 Kits ELISA
- FGF23 (Fibroblast Growth Factor 23 (FGF23))
- Autre désignation
- FGF-23 (FGF23 Produits)
- Synonymes
- FGF23 Kit ELISA, ADHR Kit ELISA, FGFN Kit ELISA, HPDR2 Kit ELISA, HYPF Kit ELISA, PHPTC Kit ELISA, fibroblast growth factor 23 Kit ELISA, fgf23 Kit ELISA, FGF23 Kit ELISA, Fgf23 Kit ELISA
- Sujet
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Gene Names: FGF23 HYPF UNQ3027/PRO9828
Protein names: Fibroblast growth factor 23 (FGF-23) (Phosphatonin) (Tumor-derived hypophosphatemia-inducing factor) [Cleaved into: Fibroblast growth factor 23 N-terminal peptide, Fibroblast growth factor 23 C-terminal peptide]
- ID gène
- 8074
- UniProt
- Q9GZV9
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion
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