JAG1 Kit ELISA

N° du produit ABIN2703276

Détail du produit JAG1 Kit ELISA

Antigène: JAG1 (Jagged 1 (JAG1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.6-400 ng/mL

Seuil minimal de détection: 1.6 ng/mL

Application: ELISA

Fonction: Human Jagged 1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Jagged 1. Other species not yet determined.

Sensibilité: 1.6 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: JAG1 (Jagged 1 (JAG1))

Autre désignation: Jagged 1 (JAG1 Produits)

Synonymes: AGS Kit ELISA, AHD Kit ELISA, AWS Kit ELISA, CD339 Kit ELISA, HJ1 Kit ELISA, JAGL1 Kit ELISA, ABE2 Kit ELISA, Gsfabe2 Kit ELISA, Htu Kit ELISA, Ozz Kit ELISA, Ser-1 Kit ELISA, JAG1 Kit ELISA, jag3 Kit ELISA, cb243 Kit ELISA, jag1 Kit ELISA, sb:cb243 Kit ELISA, serrateC Kit ELISA, jagged1 Kit ELISA, serrate Kit ELISA, serrate-1 Kit ELISA, x-serrate-1 Kit ELISA, jagged 1 Kit ELISA, jagged 1b Kit ELISA, jagged 1a Kit ELISA, jagged 1 L homeolog Kit ELISA, JAG1 Kit ELISA, Jag1 Kit ELISA, jag1 Kit ELISA, jag1b Kit ELISA, jag1a Kit ELISA, jag1.L Kit ELISA

Sujet: Gene Names: JAG1 JAGL1
Protein names: Protein jagged-1 (Jagged1) (hJ1) (CD antigen CD339)

ID gène: 182

UniProt: P78504

Pathways: Signalisation Notch, Stem Cell Maintenance