TWEAK Kit ELISA

N° du produit ABIN2703545

Détail du produit TWEAK Kit ELISA

Antigène: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 40-3.000 pg/mL

Seuil minimal de détection: 40 pg/mL

Application: ELISA

Fonction: Human TWEAK (TNFSF12) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL3, beta IG-H3, CA9, Cathepsin B, CD23, CHI3L1, CTLA-4, DKK-4, DPPIV, EDA-A2, Erythropoietin R, FGF-6, FGF-9, Gas 1, GITR, IGFBP-5, IL-1 F5 (FIL1 delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 F10 (IL-1HY2), IL-1 R5, IL-21 (IL-17C), IL-18, IL-20, IL-34, IL-5 R alpha, IL-10 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, PSMA (FOLH1), SIGIRR, TGF beta R3, Coagulation Factor III

Sensibilité: 40 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples3-20 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Autre désignation: TWEAK (TNFSF12 Produits)

Synonymes: APO3L Kit ELISA, DR3LG Kit ELISA, TWEAK Kit ELISA, Apo3l Kit ELISA, Dr3l Kit ELISA, Dr3lg Kit ELISA, Tweak Kit ELISA, TNF superfamily member 12 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 12 Kit ELISA, TNFSF12 Kit ELISA, Tnfsf12 Kit ELISA

Sujet: Gene Names: TNFSF12 APO3L DR3LG UNQ181/PRO207
Protein names: Tumor necrosis factor ligand superfamily member 12 (APO3 ligand) (TNF-related weak inducer of apoptosis) (TWEAK) [Cleaved into: Tumor necrosis factor ligand superfamily member 12, membrane form, Tumor necrosis factor ligand superfamily member 12, secreted form]

ID gène: 407977, 8742

UniProt: O43508

Pathways: Apoptose