Src Kit ELISA

N° du produit ABIN2747907

Détail du produit Src Kit ELISA

Antigène: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Épitope: pTyr419

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-SRC (Y419) ELISA Kit. This assay semi-quantitatively measures phophorylated SRC (Tyr419) in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human SRC phosphorylated at site tyrosine 419.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Autre désignation: SRC (Src Produits)

Synonymes: AW259666 Kit ELISA, pp60c-src Kit ELISA, C-src1 Kit ELISA, CG7524 Kit ELISA, D-Src64B Kit ELISA, D-src Kit ELISA, DSRC64 Kit ELISA, DSrc Kit ELISA, DSrc64 Kit ELISA, DSrc64B Kit ELISA, Dm SRC1 Kit ELISA, DmHD-358 Kit ELISA, Dmel\CG7524 Kit ELISA, Dsrc Kit ELISA, Dsrc64 Kit ELISA, Dsrc64B Kit ELISA, HD-358 Kit ELISA, MRE5 Kit ELISA, SRC 64B Kit ELISA, Src Kit ELISA, Src1 Kit ELISA, Src64 Kit ELISA, c-src Kit ELISA, dSrc Kit ELISA, dsrc Kit ELISA, src Kit ELISA, src1 Kit ELISA, src64 Kit ELISA, src64B Kit ELISA, src64b Kit ELISA, ASV Kit ELISA, SRC1 Kit ELISA, c-SRC Kit ELISA, p60-Src Kit ELISA, c-Src Kit ELISA, PP60C-SCR Kit ELISA, SDR Kit ELISA, SRC proto-oncogene, non-receptor tyrosine kinase Kit ELISA, uncharacterized LOC5574802 Kit ELISA, proto-oncogene tyrosine-protein kinase src Kit ELISA, Rous sarcoma oncogene Kit ELISA, Src oncogene at 64B Kit ELISA, SRC Kit ELISA, LOC5574802 Kit ELISA, CpipJ_CPIJ010659 Kit ELISA, Src Kit ELISA, Src64B Kit ELISA

Classe de substances: Viral Protein

Sujet: Phospho-SRC (Y419). Full Name = Proto-oncogene tyrosine-protein kinase Src (P12931)

ID gène: 6714

UniProt: P12931

Pathways: Signalistation JAK/STAT, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling