VEGFA Kit ELISA

N° du produit ABIN2748736

Détail du produit VEGFA Kit ELISA

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 40-10.000 pg/mL

Seuil minimal de détection: 40 pg/mL

Application: ELISA

Fonction: Porcine VEGF-A ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine Angiopoietin-1, IL-17F, MIF, Osteoprotegerin, PDGF-BB, RANTES, TGF-alpha, TIMP-1, TIMP-2.

Sensibilité: 40 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: VEGF-A (VEGFA Produits)

Synonymes: MVCD1 Kit ELISA, VEGF Kit ELISA, VPF Kit ELISA, Vegf Kit ELISA, Vegf120 Kit ELISA, Vegf164 Kit ELISA, Vegf188 Kit ELISA, Vpf Kit ELISA, vegf Kit ELISA, vegfa Kit ELISA, wu:fj82c06 Kit ELISA, VEGF-A Kit ELISA, VEGF164 Kit ELISA, eVEGF120 Kit ELISA, eVEGF164 Kit ELISA, vegf-a Kit ELISA, vpf Kit ELISA, vefg Kit ELISA, si:dkey-14d8.3 Kit ELISA, wu:fd42e02 Kit ELISA, vascular endothelial growth factor A Kit ELISA, vascular endothelial growth factor Aa Kit ELISA, vascular endothelial growth factor A L homeolog Kit ELISA, vascular endothelial growth factor Ab Kit ELISA, VEGFA Kit ELISA, Vegfa Kit ELISA, vegfaa Kit ELISA, vegfa.L Kit ELISA, vegfa Kit ELISA, vegfab Kit ELISA

Sujet: Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)

ID gène: 397157

UniProt: P49151

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling