HFE2 Kit ELISA

N° du produit ABIN2859238

Détail du produit HFE2 Kit ELISA

Antigène: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Épitope: AA 33-393

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Hemojuvelin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: Q33-D393

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: Q33-D393

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Muscle cell lineage. .

Plaque: Pre-coated

Protocole: mouse Hemojuvelin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Hemojuvelin has been precoated onto 96-well plates. Standards(NSO, Q33-D393) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Hemojuvelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Hemojuvelin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Hemojuvelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Hemojuvelin standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.43, Standard deviation: 0.073, CV(%): 5.1
• Sample 2: n=16, Mean(ng/ml): 3.06, Standard deviation: 0.113, CV(%): 3.7
• Sample 3: n=16, Mean(ng/ml): 5.72, Standard deviation: 0.223, CV(%): 3.9,
• Sample 1: n=24, Mean(ng/ml): 1.46, Standard deviation: 0.11, CV(%): 7.5
• Sample 2: n=24, Mean(ng/ml): 3.24, Standard deviation: 0.237, CV(%): 7.3
• Sample 3: n=24, Mean(ng/ml): 5.91, Standard deviation: 0.402, CV(%): 6.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Autre désignation: HFE2 (HFE2 Produits)

Synonymes: HFE2A Kit ELISA, HJV Kit ELISA, JH Kit ELISA, RGMC Kit ELISA, HFE2 Kit ELISA, DKFZp468F2322 Kit ELISA, 2310035L15Rik Kit ELISA, 5230400G09Rik Kit ELISA, AI414844 Kit ELISA, AI789733 Kit ELISA, DL-M Kit ELISA, Rgmc Kit ELISA, hemojuvelin Kit ELISA, RGMr Kit ELISA, hjv Kit ELISA, id:ibd3464 Kit ELISA, wu:fb38f10 Kit ELISA, zgc:136698 Kit ELISA, hemochromatosis type 2 (juvenile) Kit ELISA, hemochromatosis type 2 Kit ELISA, HFE2 Kit ELISA, Hfe2 Kit ELISA, hfe2 Kit ELISA

Sujet:

Protein Function: Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis. .

Background: Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of a three gene family (in vertebrates) called the repulsive guidance molecules. In mouse, the Hemojuvelin protein is encoded by the HFE2 gene, and it is mapped to Chromosome 3. It was concluded that downregulation of hepatic Hemojuvelin during inflammation may induce a temporary elimination of iron sensing. Hemojuvelin is a coreceptor for bone morphogenetic proteins (BMPs), and inhibition of endogenous BMP signaling reduces hepcidin expression and increases serum iron in mice. Furthermore, Hemojuvelin may play inhibitory roles in prostate cancer by suppressing cell growth, adhesion, migration and invasion.

Synonyms: Hemojuvelin,Hemochromatosis type 2 protein homolog,RGM domain family member C,Hfe2,Rgmc,

Full Gene Name: Hemojuvelin

Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor . Also released in the extracellular space..

ID gène: 69585

UniProt: Q7TQ32

Pathways: Transition Metal Ion Homeostasis