HFE2 Kit ELISA

N° du produit ABIN6720086

Détail du produit HFE2 Kit ELISA

Antigène: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: DL M,HEMOCHROMATOSIS, HEMOCHROMATOSIS DUE TO DEFECT IN HEMOJUVELIN, HEMOCHROMATOSIS DUE TO DEFECT IN HEPCIDIN ANTIMICROBIAL PEPTIDE, HEMOCHROMATOSIS JUVENILE, Hemochromatosis type 2, Hemochromatosis type 2 protein, Hemochromatosis type 2 protein homolog, HEMOCHROMATOSIS TYPE 2A, HEMOCHROMATOSIS TYPE 2B, HFE2A, HJV, JH, Juvenile, Repulsive guidance molecule c, RGM C, RGM domain family member C, RGMC

Background: Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of a three gene family (in vertebrates) called the repulsive guidance molecules. In mouse, the Hemojuvelin protein is encoded by the HFE2 gene, and it is mapped to Chromosome 3. It was concluded that downregulation of hepatic Hemojuvelin during inflammation may induce a temporary elimination of iron sensing. Hemojuvelin is a coreceptor for bone morphogenetic proteins (BMPs), and inhibition of endogenous BMP signaling reduces hepcidin expression and increases serum iron in mice. Furthermore, Hemojuvelin may play inhibitory roles in prostate cancer by suppressing cell growth, adhesion, migration and invasion.

Gene Name: HFE2

Production: Natural and recombinant mouse Hemojuvelin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q33-D393

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Hemojuvelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Hemojuvelin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Autre désignation: Hemojuvelin (HFE2 Produits)

Synonymes: HFE2A Kit ELISA, HJV Kit ELISA, JH Kit ELISA, RGMC Kit ELISA, HFE2 Kit ELISA, DKFZp468F2322 Kit ELISA, 2310035L15Rik Kit ELISA, 5230400G09Rik Kit ELISA, AI414844 Kit ELISA, AI789733 Kit ELISA, DL-M Kit ELISA, Rgmc Kit ELISA, hemojuvelin Kit ELISA, RGMr Kit ELISA, hjv Kit ELISA, id:ibd3464 Kit ELISA, wu:fb38f10 Kit ELISA, zgc:136698 Kit ELISA, hemochromatosis type 2 (juvenile) Kit ELISA, hemochromatosis type 2 Kit ELISA, HFE2 Kit ELISA, Hfe2 Kit ELISA, hfe2 Kit ELISA

ID gène: 69585

NCBI Accession: NP_081402

UniProt: Q7TQ32

Pathways: Transition Metal Ion Homeostasis