HMOX1 Kit ELISA

N° du produit ABIN2964833

Détail du produit HMOX1 Kit ELISA

Antigène: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Reactivité: Humain, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Fonction: Colorimetric detection of heme oxygenase 1

Type d'échantillon: Cell Lysate, Cyst Fluid, Serum, Tissue Samples

Analytical Method: Quantitative

Sensibilité: 0.21 ng/mL

Attributs du produit: ELISA kit used to quantitate HO-1 concentration in samples.

Ingrédients:

• Anti-HO-1 Immunoassay Plate
• 5X HO-1 Extraction Reagent
• Recombinant HO-1 Standard
• Standard and Sample Diluent
• 10X Wash Buffer Concentrate
• Anti-HO-1 Biotinylated Antibody Concentrate
• Anti-HO-1 Biotinylated Antibody Diluent
• Streptavidin: HRP Concentrate
• Streptavidin: HRP Diluent
• TMB Substrate
• Stop Solution
• Pre-treatment Buffer

Matériel non inclus: - Ultra pure water
- Additional reagents and materials for cell lysate and tissue extract preparation, including protease inhibitors
- Precision pipettors, with disposable plastic tips
- Polypropylene or polyethylene tubes to prepare samples − do not use polystyrene, polycarbonate or glass tubes
- A container to prepare 1X Wash Buffer
- A wash bottle or an automated 96-well plate washer

Information d'application

Durée du test: 0.5 h

Plaque: Pre-coated

Protocole:

1. Prepare Standard and samples in Standard and Sample Diluent.
2. Add 50 μL of Pre-Treatment Buffer to all sample and standard wells.
3. Add 50 μL of Standard and sample to appropriate wells.
4. Cover plate with Plate Sealer and incubate at room temperature (20-25 °C) for 2 hours.
5. Wash plate four times with 1X Wash Buffer.
6. Add 100 μL of Biotinylated Antibody Working Solution to each well.
7. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
8. Wash plate four times with 1X Wash Buffer as described in step
9. 9. Add 100 μL of Streptavidin-HRP Working Solution to each well.
10. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
11. Wash plate four times with 1X Wash Buffer as described in step
12. 12. Add 100 μL of TMB Substrate to each well.
13. Develop the plate in the dark at room temperature for 30 minutes.
14. Stop reaction by adding 100 μL of Stop Solution to each well.
15. Measure absorbance on a plate reader at 450 nm.

Procédure de l'essai:

1. Prepare Standard and samples in Standard and Sample Diluent.
2. Add 50 μL of Pre-Treatment Buffer to all sample and standard wells.
3. Add 50 μL of Standard and sample to appropriate wells.
4. Cover plate with Plate Sealer and incubate at room temperature (20-25 °C) for 2 hours.
5. Wash plate four times with 1X Wash Buffer.
6. Add 100 μL of Biotinylated Antibody Working Solution to each well.
7. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
8. Wash plate four times with 1X Wash Buffer as described in step
9. 9. Add 100 μL of Streptavidin-HRP Working Solution to each well.
10. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
11. Wash plate four times with 1X Wash Buffer as described in step
12. 12. Add 100 μL of TMB Substrate to each well.
13. Develop the plate in the dark at room temperature for 30 minutes.
14. Stop reaction by adding 100 μL of Stop Solution to each well.
15. Measure absorbance on a plate reader at 450 nm.

Calcul des résultats:

Duplicate absorbance values should be within 10% of each other. Care should be taken when interpreting data with differences in absorbance values greater than 10%.

1. Prepare a standard curve to determine the amount of HO-1 in an unknown sample. Plot the average absorbance obtained for each standard concentration on the vertical (Y) axis versus the corresponding HO-1 concentration on the horizontal (X) axis using graph paper or curve-fitting software.

2. Calculate the HO-1 concentration in unknown samples using the prepared standard curve. Determine the amount of HO-1 in each unknown sample by noting the HO-1 concentration (X axis) that correlates with the absorbance value (Y axis) obtained for the unknown sample.

3. Multiply the HO-1 concentration obtained by the dilution factor to determine the amount of HO-1 in the undiluted sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Autre désignation: HO-1 (HMOX1 Produits)

Synonymes: HMOX1 Kit ELISA, ARABIDOPSIS THALIANA HEME OXYGENASE 1 Kit ELISA, ATHO1 Kit ELISA, F18A8.4 Kit ELISA, F18A8_4 Kit ELISA, GENOMES UNCOUPLED 2 Kit ELISA, GUN2 Kit ELISA, HEME OXYGENASE Kit ELISA, HEME OXYGENASE 1 Kit ELISA, HEME OXYGENASE 6 Kit ELISA, HO1 Kit ELISA, HY1 Kit ELISA, HY6 Kit ELISA, PLASTID HEME OXYGENASE Kit ELISA, REVERSAL OF THE DET PHENOTYPE 4 Kit ELISA, HO-1 Kit ELISA, wu:fc27c04 Kit ELISA, zgc:65984 Kit ELISA, D8Wsu38e Kit ELISA, Hemox Kit ELISA, Hmox Kit ELISA, Hsp32 Kit ELISA, HEOXG Kit ELISA, Heox Kit ELISA, Ho-1 Kit ELISA, Ho1 Kit ELISA, hsp32 Kit ELISA, MGC132176 Kit ELISA, Hmox1 Kit ELISA, HMOX1D Kit ELISA, HSP32 Kit ELISA, bK286B10 Kit ELISA, heme oxygenase 1 Kit ELISA, Plant heme oxygenase (decyclizing) family protein Kit ELISA, Heme oxygenase Kit ELISA, heme oxygenase Kit ELISA, heme oxygenase 1, chloroplastic Kit ELISA, heme oxygenase 1a Kit ELISA, heme oxygenase 1 L homeolog Kit ELISA, HMOX1 Kit ELISA, TED4 Kit ELISA, ho1 Kit ELISA, P9301_RS17555 Kit ELISA, A9601_RS17590 Kit ELISA, MAE_RS06565 Kit ELISA, HO1 Kit ELISA, hmox1a Kit ELISA, CPE0214 Kit ELISA, Cyan7425_2962 Kit ELISA, Hmox1 Kit ELISA, hmox1.L Kit ELISA, AM1_C0205 Kit ELISA, CC1G_11686 Kit ELISA, CpipJ_CPIJ007246 Kit ELISA

Sujet: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin. These products have important physiological effects as carbon monoxide is a potent vasodilator, biliverdin and bilirubin are potent antioxidants, and the free iron increases oxidative stress and regulates the expression of many mRNAs. There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3, however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals. HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation. It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress.

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome