FAK Kit ELISA

N° du produit ABIN2748097

Détail du produit FAK Kit ELISA

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Épitope: pTyr397, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-FAK (Y397) and Total ELISA Kit. This assay semi-quantitatively measures phophorylated FAK (Tyr397) and Total FAK in lysate samples.

Type d'échantillon: Cell Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human FAK phosphorylated at site Tyrosine-397 and Total FAK.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Autre désignation: FAK (PTK2 Produits)

Synonymes: FADK Kit ELISA, FAK Kit ELISA, FAK1 Kit ELISA, FRNK Kit ELISA, PPP1R71 Kit ELISA, p125FAK Kit ELISA, pp125FAK Kit ELISA, PTK2 Kit ELISA, Fadk Kit ELISA, mKIAA4203 Kit ELISA, fak Kit ELISA, fak1a Kit ELISA, ptk2 Kit ELISA, ptk2a Kit ELISA, wu:fa18c04 Kit ELISA, zgc:66114 Kit ELISA, CG10023 Kit ELISA, CT28129 Kit ELISA, DFAK Kit ELISA, DFak Kit ELISA, DFak56 Kit ELISA, Dfak Kit ELISA, Dfak56 Kit ELISA, DmFAK Kit ELISA, Dmel\\CG10023 Kit ELISA, Fak56 Kit ELISA, Fak56D Kit ELISA, fak56 Kit ELISA, protein tyrosine kinase 2 Kit ELISA, protein tyrosine kinase 2 L homeolog Kit ELISA, PTK2 protein tyrosine kinase 2 Kit ELISA, protein tyrosine kinase 2ab Kit ELISA, Focal adhesion kinase Kit ELISA, PTK2 Kit ELISA, ptk2.L Kit ELISA, Ptk2 Kit ELISA, ptk2ab Kit ELISA, Fak Kit ELISA

Sujet: FAK phosohorylated at Tyrosine-397 and Total FAK

ID gène: 5747

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling