EGFR Kit ELISA

N° du produit ABIN4885201

Détail du produit EGFR Kit ELISA

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human EGFR SpeedELISA Kit.

Marque: SpeedELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR (EGFR Produits)

Synonymes: C-erb Kit ELISA, CG10079 Kit ELISA, D-EGFR Kit ELISA, D-Egf Kit ELISA, DEGFR Kit ELISA, DER Kit ELISA, DER flb Kit ELISA, DER/EGFR Kit ELISA, DER/faint little ball Kit ELISA, DER/top Kit ELISA, DER/torpedo Kit ELISA, DER1 Kit ELISA, DEgfr Kit ELISA, Degfr Kit ELISA, Der Kit ELISA, DmHD-33 Kit ELISA, Dmel\\CG10079 Kit ELISA, EFG-R Kit ELISA, EGF-R Kit ELISA, EGFR Kit ELISA, EGFr Kit ELISA, EGfr Kit ELISA, EK2-6 Kit ELISA, Egf Kit ELISA, Egf-r Kit ELISA, EgfR Kit ELISA, El Kit ELISA, Elp Kit ELISA, Elp-1 Kit ELISA, Elp-B1 Kit ELISA, Elp-B1RB1 Kit ELISA, HD-33 Kit ELISA, TOP Kit ELISA, Torpedo/DER Kit ELISA, Torpedo/Egfr Kit ELISA, c-erbB Kit ELISA, d-egf-r Kit ELISA, dEGFR Kit ELISA, dEGFR1 Kit ELISA, dEgfr Kit ELISA, der Kit ELISA, egfr Kit ELISA, flb Kit ELISA, l(2)05351 Kit ELISA, l(2)09261 Kit ELISA, l(2)57DEFa Kit ELISA, l(2)57EFa Kit ELISA, l(2)57Ea Kit ELISA, mor1 Kit ELISA, top Kit ELISA, top/DER Kit ELISA, top/flb Kit ELISA, torpedo/Egfr Kit ELISA, torpedo/egfr Kit ELISA, EGFR12 Kit ELISA, EGFR15 Kit ELISA, egfr1 Kit ELISA, Erbb2 Kit ELISA, ERBB Kit ELISA, ERBB1 Kit ELISA, HER1 Kit ELISA, PIG61 Kit ELISA, mENA Kit ELISA, ErbB-1 Kit ELISA, Errp Kit ELISA, 9030024J15Rik Kit ELISA, AI552599 Kit ELISA, Erbb Kit ELISA, Errb1 Kit ELISA, Wa5 Kit ELISA, wa-2 Kit ELISA, wa2 Kit ELISA, epidermal growth factor receptor Kit ELISA, Epidermal growth factor receptor Kit ELISA, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Kit ELISA, EGFR Kit ELISA, Egfr Kit ELISA, egfra Kit ELISA, egfr1 Kit ELISA, LOC5564544 Kit ELISA

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins