FGF1 Kit ELISA
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- Antigène Voir toutes FGF1 Kits ELISA
- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 12-3000 pg/mL
- Seuil minimal de détection
- 12 pg/mL
- Application
- ELISA
- Fonction
- Mouse aFGF (FGF-1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Mouse Fibroblast Growth Factor acidic (FGF1)
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes FGF1 Kits ELISA
- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
- Autre désignation
- aFGF (FGF1 Produits)
- Synonymes
- AFGF Kit ELISA, ECGF Kit ELISA, ECGF-beta Kit ELISA, ECGFA Kit ELISA, ECGFB Kit ELISA, FGF-1 Kit ELISA, FGF-alpha Kit ELISA, FGFA Kit ELISA, GLIO703 Kit ELISA, HBGF-1 Kit ELISA, HBGF1 Kit ELISA, Fgf1 Kit ELISA, FGF1 Kit ELISA, aFGF Kit ELISA, ecgf Kit ELISA, fgfa Kit ELISA, ecgfa Kit ELISA, ecgfb Kit ELISA, fgf-1 Kit ELISA, hbgf1 Kit ELISA, glio703 Kit ELISA, ecgf-beta Kit ELISA, fgf-alpha Kit ELISA, Dffrx Kit ELISA, Fam Kit ELISA, Fgf-1 Kit ELISA, Fgfa Kit ELISA, zgc:136885 Kit ELISA, LOC100221393 Kit ELISA, fgf1 Kit ELISA, zgc:73249 Kit ELISA, fibroblast growth factor 1 Kit ELISA, fibroblast growth factor 1 L homeolog Kit ELISA, fibroblast growth factor 1b Kit ELISA, fibroblast growth factor 1a Kit ELISA, FGF1 Kit ELISA, fgf1.L Kit ELISA, fgf1 Kit ELISA, Fgf1 Kit ELISA, fgf1b Kit ELISA, LOC100221393 Kit ELISA, fgf1a Kit ELISA
- ID gène
- 14164
- UniProt
- P61148
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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