FCGR2B Kit ELISA

N° du produit ABIN4885734

Détail du produit FCGR2B Kit ELISA

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Mouse Fc gamma RIIB (CD32b) SpeedELISA Kit.

Marque: SpeedELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Autre désignation: Fc gamma RIIB (FCGR2B Produits)

Synonymes: CD32 Kit ELISA, CD32B Kit ELISA, FCG2 Kit ELISA, FCGR2 Kit ELISA, IGFR2 Kit ELISA, FCGR2B Kit ELISA, AI528646 Kit ELISA, F630109E10Rik Kit ELISA, Fc[g]RII Kit ELISA, FcgRII Kit ELISA, Fcgr2 Kit ELISA, Fcgr2a Kit ELISA, Fcr-2 Kit ELISA, Fcr-3 Kit ELISA, Ly-17 Kit ELISA, Ly-m20 Kit ELISA, LyM-1 Kit ELISA, Lym-1 Kit ELISA, FCRII Kit ELISA, FcRII Kit ELISA, Fc fragment of IgG receptor IIb Kit ELISA, Fc fragment of IgG, low affinity IIb, receptor (CD32) Kit ELISA, Fc receptor, IgG, low affinity IIb Kit ELISA, low affinity immunoglobulin gamma Fc region receptor II Kit ELISA, FCGR2B Kit ELISA, Fcgr2b Kit ELISA, LOC100856270 Kit ELISA

ID gène: 14130

UniProt: P08101

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling