C4A Kit ELISA

N° du produit ABIN5067922

Détail du produit C4A Kit ELISA

Antigène: C4A (C4a) (Complement Fragment C4a (C4a))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: The Complement C4-A (C4A) ELISA Kit (Human) is a quantitative sandwich assay for the detection of C4A in human serum, plasma, tissue homogenates and other biological fluids.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: > 0.938 ng/mL

Attributs du produit: Complement C4-A (C4A) ELISA Kit (Human)

Ingrédients:

• *354394A: Microtiter Strips, 8x12 wells.
• *354394B: Standard, 2 vials
• 354394C: Sample/Standard Dilution Buffer, 1x20ml
• 354394D: Antibody-Biotin (Concentrated), 1x120ul
• 354394E: Antibody Dilution Buffer, 1x10ml
• 354394F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 354394G: SABC Dilution Buffer, 1x10ml
• 354394H: TMB Substrate, 1x10ml
• 354394J: Stop Solution, 1x10ml
• 354394K: Wash Buffer (25X), 1x30ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to C4A. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to C4A. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C4A, biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C4A in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Précision du teste: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: C4A (C4a) (Complement Fragment C4a (C4a))

Autre désignation: Complement C4-A (C4A) (C4a Produits)

Synonymes: C4 Kit ELISA, C4A2 Kit ELISA, C4A3 Kit ELISA, C4A4 Kit ELISA, C4A6 Kit ELISA, C4AD Kit ELISA, C4S Kit ELISA, CO4 Kit ELISA, CPAMD2 Kit ELISA, RG Kit ELISA, Slp Kit ELISA, C4-1 Kit ELISA, C4b Kit ELISA, complement C4A (Rodgers blood group) Kit ELISA, complement component 4A (Rodgers blood group) Kit ELISA, C4A Kit ELISA, C4a Kit ELISA

Pathways: Système du Complément