Calreticulin Kit ELISA

N° du produit ABIN5067947

Détail du produit Calreticulin Kit ELISA

Antigène: Calreticulin (CALR)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: The Human Calreticulin (CALR) ELISA Kit is a quantitative sandwich assay for the detection of CALR in human serum, plasma, tissue homogenates and other biological fluids.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.094 ng/mL

Attributs du produit: Calreticulin (CALR) ELISA Kit (Human)

Ingrédients:

• *354460A: Microtiter Strips, 8x12 wells.
• *354460B: Standard, 2 vials
• 354460C: Sample/Standard Dilution Buffer, 1x20ml
• 354460D: Antibody-Biotin (Concentrated), 1x120ul
• 354460E: Antibody Dilution Buffer, 1x10ml
• 354460F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 354460G: SABC Dilution Buffer, 1x10ml
• 354460H: TMB Substrate, 1x10ml
• 354460J: Stop Solution, 1x10ml
• 354460K: Wash Buffer (25X), 1x30ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to CALR. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to CALR. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CALR, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CALR in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Précision du teste: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: Calreticulin (CALR)

Abstract: CALR Produits

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UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response