BAFF Kit ELISA

N° du produit ABIN5652132

Détail du produit BAFF Kit ELISA

Antigène: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of B-Cell Activation Factor Receptor (BAFFR). No significant cross-reactivity or interference between B-Cell Activation Factor Receptor (BAFFR) and analogues was observed.

Sensibilité: 0.06 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Activation Factor Receptor (BAFFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell Activation Factor Receptor (BAFFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell Activation Factor Receptor (BAFFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell Activation Factor Receptor (BAFFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell Activation Factor Receptor (BAFFR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell Activation Factor Receptor (BAFFR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Autre désignation: B-Cell Activation Factor Receptor (TNFSF13B Produits)

Synonymes: BAFF Kit ELISA, BLYS Kit ELISA, CD257 Kit ELISA, DTL Kit ELISA, TALL-1 Kit ELISA, TALL1 Kit ELISA, THANK Kit ELISA, TNFSF20 Kit ELISA, ZTNF4 Kit ELISA, BAFF-R Kit ELISA, BAFFR Kit ELISA, BROMIX Kit ELISA, CD268 Kit ELISA, CVID4 Kit ELISA, prolixin Kit ELISA, TNFSF13B Kit ELISA, RGD1561519 Kit ELISA, RGD1560810 Kit ELISA, BLyS Kit ELISA, D8Ertd387e Kit ELISA, zTNF4 Kit ELISA, 2010006P15Rik Kit ELISA, Baffr Kit ELISA, Bcmd Kit ELISA, Bcmd-1 Kit ELISA, Bcmd1 Kit ELISA, Lvis22 Kit ELISA, zgc:172115 Kit ELISA, TNF superfamily member 13b Kit ELISA, TNF receptor superfamily member 13C Kit ELISA, tumor necrosis factor (ligand) superfamily, member 13b Kit ELISA, tumor necrosis factor receptor superfamily, member 13c Kit ELISA, tumor necrosis factor superfamily member 13b Kit ELISA, TNFSF13B Kit ELISA, TNFRSF13C Kit ELISA, Tnfsf13b Kit ELISA, Tnfrsf13c Kit ELISA, tnfsf13b Kit ELISA

Sujet:

Gene Name: B-Cell Activation Factor Receptor

Gene Aliases: CD268, TNFRSF13C, TNFRSF13-C, BAFF-R, BAFFR, BR3, Tumor Necrosis Factor Receptor Superfamily Member 13C

ID gène: 115650

UniProt: Q96RJ3

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response