BAFF Kit ELISA

N° du produit ABIN6719675

Détail du produit BAFF Kit ELISA

Antigène: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46.9 pg/mL - 3000 pg/mL

Seuil minimal de détection: 46.9 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: B lymphocyte stimulator, B-cell-activating factor, BAFF, BLyS, CD257, Dendritic cell-derived TNF-like molecule, soluble form, TALL-1, TN13B_HUMAN, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, Tumor necrosis factor ligand superfamily member 13b

Background: B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa.

Gene Name: TNFSF13B

Production: Natural and recombinant mouse BAFF. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A127-L309

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL mouse BAFF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse BAFF standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 46.9pg/mL-3000pg/mL

Other Performance Data: Expiration Date: 2-8° C - 06/10/2018 -20° C - 12/11/2018

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Autre désignation: BAFF (TNFSF13B Produits)

Synonymes: BAFF Kit ELISA, BLYS Kit ELISA, CD257 Kit ELISA, DTL Kit ELISA, TALL-1 Kit ELISA, TALL1 Kit ELISA, THANK Kit ELISA, TNFSF20 Kit ELISA, ZTNF4 Kit ELISA, BAFF-R Kit ELISA, BAFFR Kit ELISA, BROMIX Kit ELISA, CD268 Kit ELISA, CVID4 Kit ELISA, prolixin Kit ELISA, TNFSF13B Kit ELISA, RGD1561519 Kit ELISA, RGD1560810 Kit ELISA, BLyS Kit ELISA, D8Ertd387e Kit ELISA, zTNF4 Kit ELISA, 2010006P15Rik Kit ELISA, Baffr Kit ELISA, Bcmd Kit ELISA, Bcmd-1 Kit ELISA, Bcmd1 Kit ELISA, Lvis22 Kit ELISA, zgc:172115 Kit ELISA, TNF superfamily member 13b Kit ELISA, TNF receptor superfamily member 13C Kit ELISA, tumor necrosis factor (ligand) superfamily, member 13b Kit ELISA, tumor necrosis factor receptor superfamily, member 13c Kit ELISA, tumor necrosis factor superfamily member 13b Kit ELISA, TNFSF13B Kit ELISA, TNFRSF13C Kit ELISA, Tnfsf13b Kit ELISA, Tnfrsf13c Kit ELISA, tnfsf13b Kit ELISA

ID gène: 24099

NCBI Accession: NP_296371

UniProt: Q3KP92

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response