CD86 Kit ELISA

N° du produit ABIN5652144

Détail du produit CD86 Kit ELISA

Antigène: CD86

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of B-Lymphocyte Activation Antigen B7-2 (LAB7-2). No significant cross-reactivity or interference between B-Lymphocyte Activation Antigen B7-2 (LAB7-2) and analogues was observed.

Sensibilité: 0.274 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Activation Antigen B7-2 (LAB7-2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphocyte Activation Antigen B7-2 (LAB7-2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7-2 (LAB7-2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7-2 (LAB7-2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CD86

Autre désignation: B-Lymphocyte Activation Antigen B7-2 (CD86 Produits)

Synonymes: B7-2 Kit ELISA, B7.2 Kit ELISA, B70 Kit ELISA, CD28LG2 Kit ELISA, LAB72 Kit ELISA, B7 Kit ELISA, CLS1 Kit ELISA, Cd28l2 Kit ELISA, ETC-1 Kit ELISA, Ly-58 Kit ELISA, Ly58 Kit ELISA, MB7 Kit ELISA, MB7-2 Kit ELISA, TS/A-2 Kit ELISA, CD86 molecule Kit ELISA, CD86 antigen Kit ELISA, integrin subunit alpha 2b Kit ELISA, CD86 Kit ELISA, Cd86 Kit ELISA, Itga2b Kit ELISA

Sujet:

Gene Name: B-Lymphocyte Activation Antigen B7-2

Gene Aliases: CD86, B7-2, B70, FUN-1, CD28LG2, CD28 Antigen Ligand 2,B7-2 Antigen, CTLA-4 counter-receptor B7.2

ID gène: 942

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation