CAMK2B Kit ELISA

N° du produit ABIN5652498

Détail du produit CAMK2B Kit ELISA

Antigène: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Autre désignation: Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2B Produits)

Synonymes: CAM2 Kit ELISA, CAMK2 Kit ELISA, CAMKB Kit ELISA, Camk2d Kit ELISA, Ck2b Kit ELISA, camk2 Kit ELISA, camk2b Kit ELISA, cam2 Kit ELISA, camkb Kit ELISA, CAMK2B Kit ELISA, calcium/calmodulin dependent protein kinase II beta Kit ELISA, calcium/calmodulin-dependent protein kinase II, beta Kit ELISA, calcium/calmodulin-dependent protein kinase II beta Kit ELISA, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog Kit ELISA, calcium/calmodulin dependent protein kinase (CaM kinase) II beta L homeolog Kit ELISA, calcium/calmodulin-dependent protein kinase (CaM kinase) II beta Kit ELISA, CAMK2B Kit ELISA, Camk2b Kit ELISA, camk2a.S Kit ELISA, camk2b.L Kit ELISA

Sujet:

Gene Name: Calcium/Calmodulin Dependent Protein Kinase II Beta

Gene Aliases: CAM2, CAMK2, CAMKB, Proline Rich Calmodulin-Dependent Protein Kinase

ID gène: 816

UniProt: Q13554

Pathways: Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity